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il 1beta  (R&D Systems)


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    R&D Systems il 1beta
    Il 1beta, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 403 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+il+1beta+il+1f2/Recombinant+Human+IL-1+beta%2FIL-1F2+Protein/pm41832968-75-21-24
    Average 96 stars, based on 403 article reviews
    il 1beta - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Sterility:

    Article Title: Anti-IL-1R3 antibodies for use in inflammatory conditions
    Article Snippet: .. Materials: Plates: 384-well Low Flange White Flat Bottom Polystyrene TC-Treated Microplates Sterile; Corning; Cat. No. 3570 Proteins: IL-1 α (P026_09); Recombinant Human IL-1alpha/IL-1F1; 10 μg/mL; R&D Systems; Cat. No. 200-LA-002 IL-1 β (P026_10); Recombinant Human IL-1beta/IL-1F2; 25 μg/mL; R&D Systems; Cat. No. 200-LB-005 Standard Ab: MAB-15-0115; MAB Discovery GmbH; 2.51 mg/ml; working conc. .. 10 μg/ml Cells: A549-NFκB-RE-Luc stable transfected cells; Signosis; Cat. No. SL-0014 Medium: DMEM; PAN; Cat. No. P04-04510 FCS: Fetal Bovine Serum South Africa Low IgG; PAN; Cat. No. 1552-P120909 Pen/Strep: 10,000 U Penicillin/ml; 10 mg Streptomycin/ml; PAN Biotech; Cat. No. P06-07100 Detaching Agent: Trypsin-EDTA 1×; PAN; Cat. No. P10-023100 (4 mL for T175/2 mL for T75; ̃8 min 37° C.) Cell-Medium: DMEM, 10% FCS, 1% Pen/Strep Detection Kit: Steady-GloTM Luciferase Assay System; Promega; Cat. No. E2510 Procedure: 1.

    Article Title: Humanized anti-IL-1R3 antibodies
    Article Snippet: .. Materials: Plates: 384-well Low Flange White Flat Bottom Polystyrene TC-Treated Microplates Sterile; Corning; Cat. No. 3570 Proteins: IL-1 α (P026_09); Recombinant Human IL-1alpha/IL-1F1; 10 μg/mL; R&D Systems; Cat. No. 200-LA-002 IL-1 β (P026_10); Recombinant Human IL-1beta/IL-1F2; 25 μg/mL; R&D Systems; Cat. No. 200-LB-005 Standard Ab: MAB-15-0115; MAB Discovery GmbH; 2.51 mg/ml; working conc. .. 10 μg/ml Cells: A549-NFκB-RE-Luc stable transfected cells; Signosis; Cat. No. SL-0014 Medium: DMEM; PAN; Cat. No. P04-04510 FCS: Fetal Bovine Serum South Africa Low IgG; PAN; Cat. No. 1552-P120909 Pen/Strep: 10,000 U Penicillin/ml; 10 mg Streptomycin/ml; PAN Biotech; Cat. No. P06-07100 Detaching Agent: Trypsin-EDTA 1×; PAN; Cat. No. P10-023100 (4 mL for T175/2 mL for T75; ̃8 min 37° C.) Cell-Medium: DMEM, 10% FCS, 1% Pen/Strep Detection Kit: Steady-GloTM Luciferase Assay System; Promega; Cat. No. E2510 Procedure: 1.

    Article Title: Humanized anti-IL-1R3 antibodies
    Article Snippet: .. Materials: Plates: 384-well Low Flange White Flat Bottom Polystyrene TC-Treated Microplates Sterile; Corning; Cat. No. 3570 Proteins: IL-1 α (P026_09); Recombinant Human IL-1alpha/IL-1F1; 10 μg/mL; R&D Systems; Cat. No. 200-LA-002 IL-1 β (P026_10); Recombinant Human IL-1beta/IL-1F2; 25 μg/mL; R&D Systems; Cat. No. 200-LB-005 Standard Ab: MAB-15-0115; MAB Discovery GmbH; 2.51 mg/ml; working conc. .. 10 μg/ml Cells: A549-NFκB-RE-Luc stable transfected cells; Signosis; Cat. No. SL-0014 Medium: DMEM; PAN; Cat. No. P04-04510 FCS: Fetal Bovine Serum South Africa Low IgG; PAN; Cat. No. 1552-P120909 Pen/Strep: 10,000 U Penicillin/ml; 10 mg Streptomycin/ml; PAN Biotech; Cat. No. P06-07100 Detaching Agent: Trypsin-EDTA 1×; PAN; Cat. No. P10-023100 (4 mL for T175/2 mL for T75; ̃8 min 37° C.) Cell-Medium: DMEM, 10% FCS, 1% Pen/Strep Detection Kit: Steady-GloTM Luciferase Assay System; Promega; Cat. No. E2510 Procedure: 1.

    Recombinant:

    Article Title: Anti-IL-1R3 antibodies for use in inflammatory conditions
    Article Snippet: .. Materials: Plates: 384-well Low Flange White Flat Bottom Polystyrene TC-Treated Microplates Sterile; Corning; Cat. No. 3570 Proteins: IL-1 α (P026_09); Recombinant Human IL-1alpha/IL-1F1; 10 μg/mL; R&D Systems; Cat. No. 200-LA-002 IL-1 β (P026_10); Recombinant Human IL-1beta/IL-1F2; 25 μg/mL; R&D Systems; Cat. No. 200-LB-005 Standard Ab: MAB-15-0115; MAB Discovery GmbH; 2.51 mg/ml; working conc. .. 10 μg/ml Cells: A549-NFκB-RE-Luc stable transfected cells; Signosis; Cat. No. SL-0014 Medium: DMEM; PAN; Cat. No. P04-04510 FCS: Fetal Bovine Serum South Africa Low IgG; PAN; Cat. No. 1552-P120909 Pen/Strep: 10,000 U Penicillin/ml; 10 mg Streptomycin/ml; PAN Biotech; Cat. No. P06-07100 Detaching Agent: Trypsin-EDTA 1×; PAN; Cat. No. P10-023100 (4 mL for T175/2 mL for T75; ̃8 min 37° C.) Cell-Medium: DMEM, 10% FCS, 1% Pen/Strep Detection Kit: Steady-GloTM Luciferase Assay System; Promega; Cat. No. E2510 Procedure: 1.

    Article Title: Humanized anti-IL-1R3 antibodies
    Article Snippet: .. Materials: Assay Kit: DuoSet ELISA Human IL-6; Cat. No. DY206-05 (R&D Systems); DuoSet is consisting out of Human IL-6 Capture Antibody (part 840113), Human IL-6 Detection Antibody (part 840114), Human IL-6 (part 840115) and Streptavidin-HRP (part 8939755) Plates: 384-well clear cell culture treated plates; Corning; Cat. No. 3701 384-well Maxisorp plates; NUNC; Cat. No. 464718 PP-Plate: 120 μL 384 Deep Well “Diamond” Plate, Clear; Axygen (Corning); Cat. No. P-384-120SQ-C Proteins: IL-1 α; Recombinant Human IL-1alpha/IL-1F1; R&D Systems; Cat. No. 200-LA-002 IL-1 β; Recombinant Human IL-1beta/IL-1F2; R&D Systems; Cat. No. 200-LB-005 rhIL1-ra/IL-1F3; R&D Systems; Cat. No. 280-RA-010 Standard Abs: Human IL-1RAcP/IL-1R3 antibody; R&D Systems; Cat. No. AF676 or AF676-SP Medium: DMEM; PAN; Cat. No. P04-04510 FCS: Fetal Bovine Serum South Africa Low IgG; PAN; Cat. no. 1552-P120909 Pen/Strep: 10,000 U Penicillin/ml; 10 mg Streptomycin/ml; PAN Biotech; Cat. no. P06-07100 PBS: Buffers in a Box, Premixed PBS Buffer, 10×; Roche Applied Sciences; Cat. No. 11666789001 BSA: Bovine Serum Albumin Fraction V from bovine serum; Roche Applied Sciences; Cat. No. 10735086001 Tween 20; Tween 20; Carl Roth; Cat. No. 9127.2 TMB: TMB Solution; Invitrogen; Cat. No. SB02 HCl: 1 M Titripur Hydrochloric Acid; Merck; Cat. No. 1090571000 ELISA Buffer: PBS, 0.5% BSA, 0.05% Tween Wash Buffer: PBS, 0.1% Tween Block Buffer: PBS, 2% BSA, 0.05% Tween Cell-Medium: DMEM, 10% FCS, 1% Pen/Strep Procedure: Cell Stimulation 1. ..

    Article Title: Humanized anti-IL-1R3 antibodies
    Article Snippet: .. Materials: Assay Kit: DuoSet ELISA Human IL-6; Cat. No. DY206-05 (R&D Systems); DuoSet is consisting out of Human IL-6 Capture Antibody (part 840113), Human IL-6 Detection Antibody (part 840114), Human IL-6 (part 840115) and Streptavidin-HRP (part 8939755) Plates: 384-well clear cell culture treated plates; Corning; Cat. No. 3701 384-well Maxisorp plates; NUNC; Cat. No. 464718 PP-Plate: 120 μL 384 Deep Well “Diamond” Plate, Clear; Axygen (Corning); Cat. No. P-384-120SQ-C Proteins: IL-1 α; Recombinant Human IL-1alpha/IL-1F1; R&D Systems; Cat. No. 200-LA-002 IL-1 β; Recombinant Human IL-1beta/IL-1F2; R&D Systems; Cat. No. 200-LB-005 rhIL1-ra/IL-1F3; R&D Systems; Cat. No. 280-RA-010 Standard Abs: Human IL-1RAcP/IL-1R3 antibody; R&D Systems; Cat. No. AF676 or AF676-SP Medium: DMEM; PAN; Cat. No. P04-04510 FCS: Fetal Bovine Serum South Africa Low IgG; PAN; Cat. no. 1552-P120909 Pen/Strep: 10,000 U Penicillin/ml; 10 mg Streptomycin/ml; PAN Biotech; Cat. no. P06-07100 PBS: Buffers in a Box, Premixed PBS Buffer, 10×; Roche Applied Sciences; Cat. No. 11666789001 BSA: Bovine Serum Albumin Fraction V from bovine serum; Roche Applied Sciences; Cat. No. 10735086001 Tween 20: Tween 20; Carl Roth; Cat. No. 9127.2 TMB: TMB Solution; Invitrogen; Cat. No. SB02 HCl: 1 M Titripur Hydrochloric Acid; Merck; Cat. No. 1090571000 ELISA Buffer: PBS, 0.5% BSA, 0.05% Tween Wash Buffer: PBS, 0.1% Tween Block Buffer: PBS, 2% BSA, 0.05% Tween Cell-Medium: DMEM, 10% FCS, 1% Pen/Strep Procedure: Cell Stimulation 1. .. Plate out 6,000 A549 cells in 25 μL medium per well (conc.=2.4×105 cells/mL) to a cell culture plate.

    Article Title: Humanized anti-IL-1R3 antibodies
    Article Snippet: .. Materials: Plates: 384-well Low Flange White Flat Bottom Polystyrene TC-Treated Microplates Sterile; Corning; Cat. No. 3570 Proteins: IL-1 α (P026_09); Recombinant Human IL-1alpha/IL-1F1; 10 μg/mL; R&D Systems; Cat. No. 200-LA-002 IL-1 β (P026_10); Recombinant Human IL-1beta/IL-1F2; 25 μg/mL; R&D Systems; Cat. No. 200-LB-005 Standard Ab: MAB-15-0115; MAB Discovery GmbH; 2.51 mg/ml; working conc. .. 10 μg/ml Cells: A549-NFκB-RE-Luc stable transfected cells; Signosis; Cat. No. SL-0014 Medium: DMEM; PAN; Cat. No. P04-04510 FCS: Fetal Bovine Serum South Africa Low IgG; PAN; Cat. No. 1552-P120909 Pen/Strep: 10,000 U Penicillin/ml; 10 mg Streptomycin/ml; PAN Biotech; Cat. No. P06-07100 Detaching Agent: Trypsin-EDTA 1×; PAN; Cat. No. P10-023100 (4 mL for T175/2 mL for T75; ̃8 min 37° C.) Cell-Medium: DMEM, 10% FCS, 1% Pen/Strep Detection Kit: Steady-GloTM Luciferase Assay System; Promega; Cat. No. E2510 Procedure: 1.

    Article Title: Humanized anti-IL-1R3 antibodies
    Article Snippet: .. Materials: Plates: 384-well Low Flange White Flat Bottom Polystyrene TC-Treated Microplates Sterile; Corning; Cat. No. 3570 Proteins: IL-1 α (P026_09); Recombinant Human IL-1alpha/IL-1F1; 10 μg/mL; R&D Systems; Cat. No. 200-LA-002 IL-1 β (P026_10); Recombinant Human IL-1beta/IL-1F2; 25 μg/mL; R&D Systems; Cat. No. 200-LB-005 Standard Ab: MAB-15-0115; MAB Discovery GmbH; 2.51 mg/ml; working conc. .. 10 μg/ml Cells: A549-NFκB-RE-Luc stable transfected cells; Signosis; Cat. No. SL-0014 Medium: DMEM; PAN; Cat. No. P04-04510 FCS: Fetal Bovine Serum South Africa Low IgG; PAN; Cat. No. 1552-P120909 Pen/Strep: 10,000 U Penicillin/ml; 10 mg Streptomycin/ml; PAN Biotech; Cat. No. P06-07100 Detaching Agent: Trypsin-EDTA 1×; PAN; Cat. No. P10-023100 (4 mL for T175/2 mL for T75; ̃8 min 37° C.) Cell-Medium: DMEM, 10% FCS, 1% Pen/Strep Detection Kit: Steady-GloTM Luciferase Assay System; Promega; Cat. No. E2510 Procedure: 1.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Humanized anti-IL-1R3 antibodies
    Article Snippet: .. Materials: Assay Kit: DuoSet ELISA Human IL-6; Cat. No. DY206-05 (R&D Systems); DuoSet is consisting out of Human IL-6 Capture Antibody (part 840113), Human IL-6 Detection Antibody (part 840114), Human IL-6 (part 840115) and Streptavidin-HRP (part 8939755) Plates: 384-well clear cell culture treated plates; Corning; Cat. No. 3701 384-well Maxisorp plates; NUNC; Cat. No. 464718 PP-Plate: 120 μL 384 Deep Well “Diamond” Plate, Clear; Axygen (Corning); Cat. No. P-384-120SQ-C Proteins: IL-1 α; Recombinant Human IL-1alpha/IL-1F1; R&D Systems; Cat. No. 200-LA-002 IL-1 β; Recombinant Human IL-1beta/IL-1F2; R&D Systems; Cat. No. 200-LB-005 rhIL1-ra/IL-1F3; R&D Systems; Cat. No. 280-RA-010 Standard Abs: Human IL-1RAcP/IL-1R3 antibody; R&D Systems; Cat. No. AF676 or AF676-SP Medium: DMEM; PAN; Cat. No. P04-04510 FCS: Fetal Bovine Serum South Africa Low IgG; PAN; Cat. no. 1552-P120909 Pen/Strep: 10,000 U Penicillin/ml; 10 mg Streptomycin/ml; PAN Biotech; Cat. no. P06-07100 PBS: Buffers in a Box, Premixed PBS Buffer, 10×; Roche Applied Sciences; Cat. No. 11666789001 BSA: Bovine Serum Albumin Fraction V from bovine serum; Roche Applied Sciences; Cat. No. 10735086001 Tween 20; Tween 20; Carl Roth; Cat. No. 9127.2 TMB: TMB Solution; Invitrogen; Cat. No. SB02 HCl: 1 M Titripur Hydrochloric Acid; Merck; Cat. No. 1090571000 ELISA Buffer: PBS, 0.5% BSA, 0.05% Tween Wash Buffer: PBS, 0.1% Tween Block Buffer: PBS, 2% BSA, 0.05% Tween Cell-Medium: DMEM, 10% FCS, 1% Pen/Strep Procedure: Cell Stimulation 1. ..

    Article Title: Humanized anti-IL-1R3 antibodies
    Article Snippet: .. Materials: Assay Kit: DuoSet ELISA Human IL-6; Cat. No. DY206-05 (R&D Systems); DuoSet is consisting out of Human IL-6 Capture Antibody (part 840113), Human IL-6 Detection Antibody (part 840114), Human IL-6 (part 840115) and Streptavidin-HRP (part 8939755) Plates: 384-well clear cell culture treated plates; Corning; Cat. No. 3701 384-well Maxisorp plates; NUNC; Cat. No. 464718 PP-Plate: 120 μL 384 Deep Well “Diamond” Plate, Clear; Axygen (Corning); Cat. No. P-384-120SQ-C Proteins: IL-1 α; Recombinant Human IL-1alpha/IL-1F1; R&D Systems; Cat. No. 200-LA-002 IL-1 β; Recombinant Human IL-1beta/IL-1F2; R&D Systems; Cat. No. 200-LB-005 rhIL1-ra/IL-1F3; R&D Systems; Cat. No. 280-RA-010 Standard Abs: Human IL-1RAcP/IL-1R3 antibody; R&D Systems; Cat. No. AF676 or AF676-SP Medium: DMEM; PAN; Cat. No. P04-04510 FCS: Fetal Bovine Serum South Africa Low IgG; PAN; Cat. no. 1552-P120909 Pen/Strep: 10,000 U Penicillin/ml; 10 mg Streptomycin/ml; PAN Biotech; Cat. no. P06-07100 PBS: Buffers in a Box, Premixed PBS Buffer, 10×; Roche Applied Sciences; Cat. No. 11666789001 BSA: Bovine Serum Albumin Fraction V from bovine serum; Roche Applied Sciences; Cat. No. 10735086001 Tween 20: Tween 20; Carl Roth; Cat. No. 9127.2 TMB: TMB Solution; Invitrogen; Cat. No. SB02 HCl: 1 M Titripur Hydrochloric Acid; Merck; Cat. No. 1090571000 ELISA Buffer: PBS, 0.5% BSA, 0.05% Tween Wash Buffer: PBS, 0.1% Tween Block Buffer: PBS, 2% BSA, 0.05% Tween Cell-Medium: DMEM, 10% FCS, 1% Pen/Strep Procedure: Cell Stimulation 1. .. Plate out 6,000 A549 cells in 25 μL medium per well (conc.=2.4×105 cells/mL) to a cell culture plate.

    Cell Culture:

    Article Title: Humanized anti-IL-1R3 antibodies
    Article Snippet: .. Materials: Assay Kit: DuoSet ELISA Human IL-6; Cat. No. DY206-05 (R&D Systems); DuoSet is consisting out of Human IL-6 Capture Antibody (part 840113), Human IL-6 Detection Antibody (part 840114), Human IL-6 (part 840115) and Streptavidin-HRP (part 8939755) Plates: 384-well clear cell culture treated plates; Corning; Cat. No. 3701 384-well Maxisorp plates; NUNC; Cat. No. 464718 PP-Plate: 120 μL 384 Deep Well “Diamond” Plate, Clear; Axygen (Corning); Cat. No. P-384-120SQ-C Proteins: IL-1 α; Recombinant Human IL-1alpha/IL-1F1; R&D Systems; Cat. No. 200-LA-002 IL-1 β; Recombinant Human IL-1beta/IL-1F2; R&D Systems; Cat. No. 200-LB-005 rhIL1-ra/IL-1F3; R&D Systems; Cat. No. 280-RA-010 Standard Abs: Human IL-1RAcP/IL-1R3 antibody; R&D Systems; Cat. No. AF676 or AF676-SP Medium: DMEM; PAN; Cat. No. P04-04510 FCS: Fetal Bovine Serum South Africa Low IgG; PAN; Cat. no. 1552-P120909 Pen/Strep: 10,000 U Penicillin/ml; 10 mg Streptomycin/ml; PAN Biotech; Cat. no. P06-07100 PBS: Buffers in a Box, Premixed PBS Buffer, 10×; Roche Applied Sciences; Cat. No. 11666789001 BSA: Bovine Serum Albumin Fraction V from bovine serum; Roche Applied Sciences; Cat. No. 10735086001 Tween 20; Tween 20; Carl Roth; Cat. No. 9127.2 TMB: TMB Solution; Invitrogen; Cat. No. SB02 HCl: 1 M Titripur Hydrochloric Acid; Merck; Cat. No. 1090571000 ELISA Buffer: PBS, 0.5% BSA, 0.05% Tween Wash Buffer: PBS, 0.1% Tween Block Buffer: PBS, 2% BSA, 0.05% Tween Cell-Medium: DMEM, 10% FCS, 1% Pen/Strep Procedure: Cell Stimulation 1. ..

    Article Title: Humanized anti-IL-1R3 antibodies
    Article Snippet: .. Materials: Assay Kit: DuoSet ELISA Human IL-6; Cat. No. DY206-05 (R&D Systems); DuoSet is consisting out of Human IL-6 Capture Antibody (part 840113), Human IL-6 Detection Antibody (part 840114), Human IL-6 (part 840115) and Streptavidin-HRP (part 8939755) Plates: 384-well clear cell culture treated plates; Corning; Cat. No. 3701 384-well Maxisorp plates; NUNC; Cat. No. 464718 PP-Plate: 120 μL 384 Deep Well “Diamond” Plate, Clear; Axygen (Corning); Cat. No. P-384-120SQ-C Proteins: IL-1 α; Recombinant Human IL-1alpha/IL-1F1; R&D Systems; Cat. No. 200-LA-002 IL-1 β; Recombinant Human IL-1beta/IL-1F2; R&D Systems; Cat. No. 200-LB-005 rhIL1-ra/IL-1F3; R&D Systems; Cat. No. 280-RA-010 Standard Abs: Human IL-1RAcP/IL-1R3 antibody; R&D Systems; Cat. No. AF676 or AF676-SP Medium: DMEM; PAN; Cat. No. P04-04510 FCS: Fetal Bovine Serum South Africa Low IgG; PAN; Cat. no. 1552-P120909 Pen/Strep: 10,000 U Penicillin/ml; 10 mg Streptomycin/ml; PAN Biotech; Cat. no. P06-07100 PBS: Buffers in a Box, Premixed PBS Buffer, 10×; Roche Applied Sciences; Cat. No. 11666789001 BSA: Bovine Serum Albumin Fraction V from bovine serum; Roche Applied Sciences; Cat. No. 10735086001 Tween 20: Tween 20; Carl Roth; Cat. No. 9127.2 TMB: TMB Solution; Invitrogen; Cat. No. SB02 HCl: 1 M Titripur Hydrochloric Acid; Merck; Cat. No. 1090571000 ELISA Buffer: PBS, 0.5% BSA, 0.05% Tween Wash Buffer: PBS, 0.1% Tween Block Buffer: PBS, 2% BSA, 0.05% Tween Cell-Medium: DMEM, 10% FCS, 1% Pen/Strep Procedure: Cell Stimulation 1. .. Plate out 6,000 A549 cells in 25 μL medium per well (conc.=2.4×105 cells/mL) to a cell culture plate.

    Blocking Assay:

    Article Title: Humanized anti-IL-1R3 antibodies
    Article Snippet: .. Materials: Assay Kit: DuoSet ELISA Human IL-6; Cat. No. DY206-05 (R&D Systems); DuoSet is consisting out of Human IL-6 Capture Antibody (part 840113), Human IL-6 Detection Antibody (part 840114), Human IL-6 (part 840115) and Streptavidin-HRP (part 8939755) Plates: 384-well clear cell culture treated plates; Corning; Cat. No. 3701 384-well Maxisorp plates; NUNC; Cat. No. 464718 PP-Plate: 120 μL 384 Deep Well “Diamond” Plate, Clear; Axygen (Corning); Cat. No. P-384-120SQ-C Proteins: IL-1 α; Recombinant Human IL-1alpha/IL-1F1; R&D Systems; Cat. No. 200-LA-002 IL-1 β; Recombinant Human IL-1beta/IL-1F2; R&D Systems; Cat. No. 200-LB-005 rhIL1-ra/IL-1F3; R&D Systems; Cat. No. 280-RA-010 Standard Abs: Human IL-1RAcP/IL-1R3 antibody; R&D Systems; Cat. No. AF676 or AF676-SP Medium: DMEM; PAN; Cat. No. P04-04510 FCS: Fetal Bovine Serum South Africa Low IgG; PAN; Cat. no. 1552-P120909 Pen/Strep: 10,000 U Penicillin/ml; 10 mg Streptomycin/ml; PAN Biotech; Cat. no. P06-07100 PBS: Buffers in a Box, Premixed PBS Buffer, 10×; Roche Applied Sciences; Cat. No. 11666789001 BSA: Bovine Serum Albumin Fraction V from bovine serum; Roche Applied Sciences; Cat. No. 10735086001 Tween 20; Tween 20; Carl Roth; Cat. No. 9127.2 TMB: TMB Solution; Invitrogen; Cat. No. SB02 HCl: 1 M Titripur Hydrochloric Acid; Merck; Cat. No. 1090571000 ELISA Buffer: PBS, 0.5% BSA, 0.05% Tween Wash Buffer: PBS, 0.1% Tween Block Buffer: PBS, 2% BSA, 0.05% Tween Cell-Medium: DMEM, 10% FCS, 1% Pen/Strep Procedure: Cell Stimulation 1. ..

    Article Title: Humanized anti-IL-1R3 antibodies
    Article Snippet: .. Materials: Assay Kit: DuoSet ELISA Human IL-6; Cat. No. DY206-05 (R&D Systems); DuoSet is consisting out of Human IL-6 Capture Antibody (part 840113), Human IL-6 Detection Antibody (part 840114), Human IL-6 (part 840115) and Streptavidin-HRP (part 8939755) Plates: 384-well clear cell culture treated plates; Corning; Cat. No. 3701 384-well Maxisorp plates; NUNC; Cat. No. 464718 PP-Plate: 120 μL 384 Deep Well “Diamond” Plate, Clear; Axygen (Corning); Cat. No. P-384-120SQ-C Proteins: IL-1 α; Recombinant Human IL-1alpha/IL-1F1; R&D Systems; Cat. No. 200-LA-002 IL-1 β; Recombinant Human IL-1beta/IL-1F2; R&D Systems; Cat. No. 200-LB-005 rhIL1-ra/IL-1F3; R&D Systems; Cat. No. 280-RA-010 Standard Abs: Human IL-1RAcP/IL-1R3 antibody; R&D Systems; Cat. No. AF676 or AF676-SP Medium: DMEM; PAN; Cat. No. P04-04510 FCS: Fetal Bovine Serum South Africa Low IgG; PAN; Cat. no. 1552-P120909 Pen/Strep: 10,000 U Penicillin/ml; 10 mg Streptomycin/ml; PAN Biotech; Cat. no. P06-07100 PBS: Buffers in a Box, Premixed PBS Buffer, 10×; Roche Applied Sciences; Cat. No. 11666789001 BSA: Bovine Serum Albumin Fraction V from bovine serum; Roche Applied Sciences; Cat. No. 10735086001 Tween 20: Tween 20; Carl Roth; Cat. No. 9127.2 TMB: TMB Solution; Invitrogen; Cat. No. SB02 HCl: 1 M Titripur Hydrochloric Acid; Merck; Cat. No. 1090571000 ELISA Buffer: PBS, 0.5% BSA, 0.05% Tween Wash Buffer: PBS, 0.1% Tween Block Buffer: PBS, 2% BSA, 0.05% Tween Cell-Medium: DMEM, 10% FCS, 1% Pen/Strep Procedure: Cell Stimulation 1. .. Plate out 6,000 A549 cells in 25 μL medium per well (conc.=2.4×105 cells/mL) to a cell culture plate.

    Cell Stimulation:

    Article Title: Humanized anti-IL-1R3 antibodies
    Article Snippet: .. Materials: Assay Kit: DuoSet ELISA Human IL-6; Cat. No. DY206-05 (R&D Systems); DuoSet is consisting out of Human IL-6 Capture Antibody (part 840113), Human IL-6 Detection Antibody (part 840114), Human IL-6 (part 840115) and Streptavidin-HRP (part 8939755) Plates: 384-well clear cell culture treated plates; Corning; Cat. No. 3701 384-well Maxisorp plates; NUNC; Cat. No. 464718 PP-Plate: 120 μL 384 Deep Well “Diamond” Plate, Clear; Axygen (Corning); Cat. No. P-384-120SQ-C Proteins: IL-1 α; Recombinant Human IL-1alpha/IL-1F1; R&D Systems; Cat. No. 200-LA-002 IL-1 β; Recombinant Human IL-1beta/IL-1F2; R&D Systems; Cat. No. 200-LB-005 rhIL1-ra/IL-1F3; R&D Systems; Cat. No. 280-RA-010 Standard Abs: Human IL-1RAcP/IL-1R3 antibody; R&D Systems; Cat. No. AF676 or AF676-SP Medium: DMEM; PAN; Cat. No. P04-04510 FCS: Fetal Bovine Serum South Africa Low IgG; PAN; Cat. no. 1552-P120909 Pen/Strep: 10,000 U Penicillin/ml; 10 mg Streptomycin/ml; PAN Biotech; Cat. no. P06-07100 PBS: Buffers in a Box, Premixed PBS Buffer, 10×; Roche Applied Sciences; Cat. No. 11666789001 BSA: Bovine Serum Albumin Fraction V from bovine serum; Roche Applied Sciences; Cat. No. 10735086001 Tween 20; Tween 20; Carl Roth; Cat. No. 9127.2 TMB: TMB Solution; Invitrogen; Cat. No. SB02 HCl: 1 M Titripur Hydrochloric Acid; Merck; Cat. No. 1090571000 ELISA Buffer: PBS, 0.5% BSA, 0.05% Tween Wash Buffer: PBS, 0.1% Tween Block Buffer: PBS, 2% BSA, 0.05% Tween Cell-Medium: DMEM, 10% FCS, 1% Pen/Strep Procedure: Cell Stimulation 1. ..

    Article Title: Humanized anti-IL-1R3 antibodies
    Article Snippet: .. Materials: Assay Kit: DuoSet ELISA Human IL-6; Cat. No. DY206-05 (R&D Systems); DuoSet is consisting out of Human IL-6 Capture Antibody (part 840113), Human IL-6 Detection Antibody (part 840114), Human IL-6 (part 840115) and Streptavidin-HRP (part 8939755) Plates: 384-well clear cell culture treated plates; Corning; Cat. No. 3701 384-well Maxisorp plates; NUNC; Cat. No. 464718 PP-Plate: 120 μL 384 Deep Well “Diamond” Plate, Clear; Axygen (Corning); Cat. No. P-384-120SQ-C Proteins: IL-1 α; Recombinant Human IL-1alpha/IL-1F1; R&D Systems; Cat. No. 200-LA-002 IL-1 β; Recombinant Human IL-1beta/IL-1F2; R&D Systems; Cat. No. 200-LB-005 rhIL1-ra/IL-1F3; R&D Systems; Cat. No. 280-RA-010 Standard Abs: Human IL-1RAcP/IL-1R3 antibody; R&D Systems; Cat. No. AF676 or AF676-SP Medium: DMEM; PAN; Cat. No. P04-04510 FCS: Fetal Bovine Serum South Africa Low IgG; PAN; Cat. no. 1552-P120909 Pen/Strep: 10,000 U Penicillin/ml; 10 mg Streptomycin/ml; PAN Biotech; Cat. no. P06-07100 PBS: Buffers in a Box, Premixed PBS Buffer, 10×; Roche Applied Sciences; Cat. No. 11666789001 BSA: Bovine Serum Albumin Fraction V from bovine serum; Roche Applied Sciences; Cat. No. 10735086001 Tween 20: Tween 20; Carl Roth; Cat. No. 9127.2 TMB: TMB Solution; Invitrogen; Cat. No. SB02 HCl: 1 M Titripur Hydrochloric Acid; Merck; Cat. No. 1090571000 ELISA Buffer: PBS, 0.5% BSA, 0.05% Tween Wash Buffer: PBS, 0.1% Tween Block Buffer: PBS, 2% BSA, 0.05% Tween Cell-Medium: DMEM, 10% FCS, 1% Pen/Strep Procedure: Cell Stimulation 1. .. Plate out 6,000 A549 cells in 25 μL medium per well (conc.=2.4×105 cells/mL) to a cell culture plate.



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